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| 鸡蛋中残留四环素类抗生素的快速测定研究 |
| Trace Determination of Tetracyclines in Eggs by Capillary Electrophoresis with Electrochemical Detection |
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| DOI：10.3969/j.issn.1671-5322.2006.04.005 |
| 中文关键词: 四环素 土霉素 毛细管电泳 |
| 英文关键词: tetracyclines oxytetracycline capillary electrophoresis |
| 基金项目: |
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| 中文摘要: |
| 主要运用毛细管电泳-电化学检测法(CE-ED)对鸡蛋中残留的四环素(Tetracycline,TC)、土霉素(Oxytetracycline,OTC)进行快速测定分析.实验研究表明,甲醇的加入量为样品体积的一半时,基本能消除鸡蛋蛋白的影响;在检测电位为1.2 V,20 mmol/L缓冲溶液(pH为2.6),25 kV分离电压,进样8 s的条件下,TC和OTC有很好的分离效果.TC和OTC的线性范围分别为:5.0×10-4～2.0×10-6g/mL和3.0×10-4-8.0×10-6g/mL,检测限分别为1.9×10-8、4.1×10-8g/mL,结果令人满意. |
| 英文摘要: |
| A capillary electrophoresis technique with electrochemical detection（CE-ED） for determination of tetracyclines（TC） and oxytetracycline（OTC） in eggs was described in this paper.Effects of several factors,such as the pH value and concentration of the running buffer,the concentration of Tween-20,separation voltage,injection time and the potential applied to the working electrode,were investigated to find the optimum conditions.Well-defined separation of TC and OTC was achieved in the running sodium phosphate monobasic（pH 2.6）.Operated in a wall-jet configuration,a carbon-disk electrode used as the working electrode exhibits good response at +1.20 V（vs.SCE） for the analytes.The detection limits（S/N=3）for TC,OTC was 1.9×10-8 g/ml,4.1×10-8 g/ml,respecratively. |
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