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| 黄鳝SOD,AKP,补体C3、C4组织分布研究 |
| Tisssue Distribution of the Superoxide Dismutase, Phosphatases and Complement Components in Monopterus Ablus |
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| DOI:10.3969/j.issn.1671-5322.2012.02.015 |
| 中文关键词: 黄鳝 非特异性免疫酶 组织分布 |
| 英文关键词: Monopterus ablus SOD AKP ACK complement components tissue distribution |
| 基金项目:十一·五科技支撑计划项目,公益性行业专项,湖南农业大学人才引进项目 |
| 作者 | 单位 | | 郇志利 | 湖南农业大学动物科学技术学院,湖南长沙,410128 | | 毛盼 | 湖南农业大学动物科学技术学院,湖南长沙,410128 | | 何蓝波 | 四川农业大学动物科技学院,四川雅安,625000 | | 胡毅 | 湖南农业大学动物科学技术学院,湖南长沙410128 益阳益华水产品有限公司,湖南益阳413000 | | 黄云 | 湖南农业大学动物科学技术学院,湖南长沙,410128 | | 戴振炎 | 湖南农业大学动物科学技术学院,湖南长沙,410128 |
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| 中文摘要: |
| 以在水温28±3℃下饲养的黄鳝(Monopterus ablus)(37.35±0.89g)为研究对象,以酶学方法研究黄鳝不同免疫组织(血液、体表粘液、肝脏、肠道、肾脏、脾脏)中非特异性免疫相关酶(超氧化物歧化酶(SOD)、酸性和碱性磷酸酶(ACP、AKP)、补体C3、C4)的分布情况。研究结果显示,组织中SOD活性依次为;肝脏〉肾脏、脾脏〉粘液〉肠道〉血液(P〈0.05):ACP活性依次为:脾脏〉肾脏〉肝脏〉肠道〉血液、粘液;AKP活性依次为:肾脏〉肝脏〉脾脏〉肠道〉粘液〉血液;补体C3的含量依次为:肝脏〉肾脏〉脾脏〉肠道〉血液、粘液;补体C4的含量依次为:肝脏〉脾脏〉肾脏〉肠道〉血液、粘液。由此可见,超氧化物歧化酶和补体C3、C4主要分布于肝脏中,碱性磷酸酶主要分布于肾脏中,酸性磷酸酶主要分布于脾脏中;而各种非特异性酶和物质在肠道、血液和粘液中也均有少量分布。 |
| 英文摘要: |
| The Monopterus ablus(average weight: 37.35±0.89 g) were bred in the temperature of 28±3 ℃.The tissue distribution of immune-related enzymes including the superoxide dismutase(SOD),alkaline phosphatase(AKP),acid phosphatase(ACP),complement components C3 and C4 were examined with enzyme analytical method.The results showed that: The activity of SOD in different tissues was liver>kidney>spleen>mucus>Intestine>blood;the activity of ACP ranged as follows: spleen>kidney>liver> intestine>blood>mucus;the activity of AKP ranged as:kidney>liver>spleen >intestine>mucus>blood,the content of complement C3 was liver>kidney>spleen>intestine>blood >mucus,and complement C4 was liver>spleen> kidney>intestine> blood>mucus.Those results indicated that the activities of SOD,ACP,AKP and the content of complement components(C3,C4) in liver,kidney,spleen was higher than that of other tissues. |
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