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| 功能化多壁碳纳米管修饰的葡萄糖传感器的研究 |
| Glucose Sensor Based on Potassium-doped Multi-walled Carbon Nanotubes |
| 投稿时间：2015-08-31 |
| DOI：10.16018/j.cnki.cn32-1650/n.201504012功能化多壁碳纳米管修饰的葡萄糖传感器的研究 |
| 中文关键词: 多壁碳纳米管 钾 葡萄糖氧化酶 电化学 |
| 英文关键词: multi-walled carbon nanotubes potassium glucose oxidase electrochemistry |
| 基金项目:教育部人文社会科学青年基金项目(13YJC751080) |
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| 中文摘要: |
| 在室温下利用化学掺杂法合成了K掺杂多壁碳纳米管KMWNTs，通过固定葡萄糖氧化酶(GOx)在KMWNTs修饰的玻碳电极表面，并利用葡萄糖氧化酶(GOx)的直接电化学，构建了一种新型葡萄糖传感器。利用扫描电镜对MWNTs和KMWNTs的形貌进行表征发现，K掺杂后没有破坏MWNTs的管状结构；采用电化学系统对传感器的性质进行了研究，结果表明，与单一的MWNTs相比，KMWNTs显示了更为有效的电催化活性。在此基础上，以KMWNTs膜为基底构建了抗干扰能力强、稳定性好、灵敏度高、响应快的葡萄糖传感器，在-0.52 V的检测电位下，该传感器对葡萄糖响应的线性范围为0.1~3.0 mmol·L-1(R=0.998)，检测限为0.02 mmol·L-1(S/N=3)，常见干扰物质如抗坏血酸和尿酸的存在不影响测定。 |
| 英文摘要: |
| The potassium-doped multi-walled carbon nanotubes(KMWNTs)were synthesized according to the chemical K doping at room temperature. We demonstrate herein a newly developed amperometric glucose biosensor by using the direct electron transfer of glucose oxidase(GOx), which is base on the immobilization of GOx on KMWNTs modified electrodes. The structures of the MWNTs and KMWNTs were characterized by scanning electron microscopy( SEM). After K-doping, no disruption of MWNTs morphology is observed. As a new platform in glucose analysis, the resulting electrode(GOx/KMWNTs/GCE)exhibits a sensitive response to glucose, with a linear range from 0. 1 to 3. 0 mmol·L-1(R=0. 998)and a detection limit of 0. 02 mmol·L-1(S/N =3). At an applied potential of -0. 52 V, the resulting biosensor performs a sensitive and selective electrochemical response to glucose in the presence of common interferences, such as ascorbic acid(AA)and uric acid(UA). These results indicated that the GOx/KMWNTs modified glassy carbon electrode presents stable, high sensitivity and also exhibits fast amperometric response to the detection of glucose, which is promising for the development of glucose sensor. |
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