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| 双电位比率型量子点ECL适配体传感器的构建及其应用 |
| Construction and Application of Dual Potential Ratio-dependent Quantum Dot ECL Aptamer Sensor |
| 投稿时间：2021-03-17 |
| DOI：10.16018/j.cnki.cn32-1650/n.202102013 |
| 中文关键词: 比率型电致化学发光 适配体传感器 硒化镉量子点 钌硅纳米粒子 抗生素 |
| 英文关键词: ratio-dependent ECL aptamer sensor CdSe QDs ruthenium silicon nanoparticles antibiotic |
| 基金项目:国家自然科学基金(21505001)；安徽省科技重大专项(201903a06020003)。 |
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| 中文摘要: |
| 比率型电致化学发光适配体传感器检测目标时可显示两种不同的ECL信号，以往对比率型ECL传感的研究大多基于鲁米诺-过氧化氢(Luminol-H2O2)体系。研究设计了一种基于硒化镉量子点(CdSe QDs)和钌联吡啶修饰的钌硅纳米粒子(RuSi@Ru(bpy)2+3 NPs)的双电位比率型ECL适配体传感器，用于卡那霉素超灵敏高选择性检测。分别选取CdSe QDs和RuSi@Ru(bpy)2+3 NPs作为阴极和阳极ECL发光体。通过卡那霉素两条互补的适配体链作为连接CdSe QDs与RuSi@Ru(bpy)2+3 NPs的媒介，通过酰胺偶联反应在玻碳电极表面构建检测卡那霉素传感器。卡那霉素检测浓度范围为1.0 fmol/L~10 pmol/L。线性拟合方程为△I=0.132 lg c+2.133，相关系数为0.999(c代表卡那霉素浓度，mol/L)。信噪比S/N=3时，检测限(LOD)为0.4 fmol/L。研究结果为两个ECL发光体构建比率型ECL传感器提供了新思路。 |
| 英文摘要: |
| Ratio-dependent electrochemiluminescence aptamer sensors can display two different ECL signals when detecting targets. Most previous studies on ratio-dependent ECL sensing are based on the Luminol-hydrogen peroxide system. A two-potential ratio ECL aptamer sensor based on cadmium selenide quantum dots(CdSe QDs)and ruthenium-bipyridine modified Ru-Si nanoparticles(RuSi@Ru(bpy)2+3 NPs)was designed for the ultra-sensitive and highly selective detection of kanamycin. CdSe QDs and RuSi@Ru(bpy)2+3 NPs were selected as cathode and anode ECL emitters, respectively. Two complementary aptamer chains of kanamycin are used as the medium for connecting CdSe QDs and RuSi@Ru(bpy)2+3 NPs, and a kanamycin sensor is constructed on the surface of glassy carbon electrode through amide coupling reaction. The detection concentration of kanamycin ranged from 1. 0 fmol/L to 10 pmol/L. The linear fitting equation was △I=0. 132 lg c+2. 133, and the correlation coefficient was 0. 999(C represents the concentration of kanamycin, mol/L). When the signal-to-noise ratio S/N=3, the detection limit(LOD)is 0. 4 fmol/L. The research results provide a new idea for constructing a ratio-dependent ECL sensor with two ECL emitter. |
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