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| HPLC测定苦参配方颗粒中苦参碱与氧化苦参碱含量 |
| Simultaneous Determination of Alkali and Alkali Oxides in Kushen Recipe Granule with HPLC |
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| DOI：10.3969/j.issn.1671-5322.2013.01.012 |
| 中文关键词: 苦参配方颗粒 HPLC 苦参碱 氧化苦参碱 |
| 英文关键词: Kushen recipe granules HPLC Matrine Oxymatrine |
| 基金项目: |
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| 中文摘要: |
| 采用高效液相色谱法,以氨基键合硅胶为填充剂,以乙腈-无水乙醇-3％磷酸溶液(80∶10∶10)为流动相,检测波长为220 nm,建立了一种可同时测定苦参配方颗粒中苦参碱与氧化苦参碱含量的方法.结果表明:苦参碱回归方程为Y=499.412 2X-8.070 1,R=0.999 9,线性范围为0.158 ～1.580μg.氧化苦参碱回归方程为Y=557.152 4X-15.568 8,R=0.999 9,线性范围为0.1 68 6～1.686 0μg.苦参配方颗粒中苦参碱和氧化苦参碱的总量的平均含量为4.60％,回收率在98％ ～103％之间,RSD为1.52％. |
| 英文摘要: |
| Objective: To develop a RP-HPLC method for simultaneous determination of Matrine and Oxymatrine.Methods: Using high performance liquid chromatography,amino-bonded silica gel as a filling agent;Acetonitrile-ethanol-3% phosphate solution(80:10:10) for the mobile phase;Detection wavelength of 220 nm.Results: regression equations for Matrine Y=499.4122X-8.070 1,R=0.999 9,linear range from 0.158~1.58 μg.Oxymatrine regression equation for Y=557.152 4X-15.568 8,R=0.999 9,linear range 0.168 6~1.686 μg.Kushen recipe granules of Matrine and Oxymatrine in the total amount of the average content of 4.60%,the recovery rate between in 98% and 103%,RSD of 1.52%.Conclusion:The method could determine Matrine and Oxymatrine in Kushen recipe granule. |
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